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KMID : 0363320110320040519
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Journal of Korean Oriental Internal Medicine
2011 Volume.32 No. 4 p.519 ~ p.529
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The effects of Injinchunggan-tang on Cell Growth and Apoptosis in Human Hepatic Stellate Cell Line LX2
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Kim Sang-Joo
Woo Hong-Jung
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Abstract
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Objectives: This study was performed to investigate the effects of Injinchunggan-tang on cell growth and apoptosis in human hepatic stellate cell line LX2.
Materials and Methods : Hepatic stellate cells were treated with various concentrations of Injinchunggan-tang extract for 24, 48 and 72 hours. The extraction was done with distilled water. After the treatment, cell viability, proliferation, apoptosis, caspase activity, caspase inhibitor and the mRNA of the Bcl-2, and Bax with β-actin were measured by using MTT assay, apoptosis assay and RT-PCR.
Results: Proliferation, and mRNA expression of the hepatic stellate cells were inhibited by Injinchunggan-tang treatment in a dose-dependent manner. This indicates the prescription has inhibitory effect on fibrogenesis of the liver by regulating the fibrogenesis-associated genes in transcription. Cell viability was inhibited in time- and dose-dependent manners.
Conclusions: These results suggest that Injinchunggan-tang would be beneficial in the treatment of cirrhotic patients as well as for the patients with chronic hepatitis.
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KEYWORD
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Injinchunggan-tang (Yinchenqinggan-tang), human hepatic stellate cells LX2, apoptosis, caspase inhibitor
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